User:Megan L. Channell/Notebook/Horseradish/2013/09/11

Biomaterials Design Lab

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Horseradish Peroxidase UV-Vis and Gold Solution Objective Make more standards of HRP and run on UV-Vis Test gold standards, citrate-AuNP solutions and BSA-AuNP solutions on the AA/ICPMS Protocol The protocol can be found from yesterdays lab. The only adjestment was that the diltuion of the HRP was a total of 10 mL instead of 2mL. Data AA/ICPMS of own personal recordings Calibration curve for Gold on own personal standards Calibration Curve of class data for Gold on AA/ICPMS UV-Vis Trial 3 of HRP Calibration Curve of HRP for trial 3 Calculations Determine how many atoms are in a AuNP Avg. abs. for citrate was .1444 Use the calibration curve on group data, y=3×10-5x2+0.0209x .1444=3×10-5x2+0.0209x Solve for x x= -703.51 and x=6.842 So, x= 6.842μg/mL The concentration we used was diluted by a factor of 10. So actual concentration is .06842g/L .006842g/L Au* (1 mol/ 196.96657 g Au)= 3.47x10-4 M of Au The #Au Atoms/NP = [Au]/[citrate-AuNP] = (3.47x10-4 M)/( 9.17×10-9M)= 37,884 The concentration of citrate AuNP was found in a previous lab. The amount of Au atoms in citrate-AuNP is 37,884