User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/18

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Entry title Make polydopamine for nanofibers Myoglobin for procedure Procedure Preparing scaffolds Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour First two scaffolds immersed in solution pH 8.51 Second two scaffolds immersed in solution pH 8.59 Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours Rinse with deionized water and allow scaffold to dry overnight Results Brute force Scaffolds after 1 hr in Tris-polydopamine Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution