User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/22

Cellular Adhesion

• No PCR Supermix until 2pm

• PCR
  • Template: 40ul PCR Supermix, .8ul primers (.4ul primers for GCN4), .8ul DNA (only GCN4)
    • 6His Tag: 6His_Tag-F and 6His_Tag-R
    • FLAG Tag: FLAG_Tag-F and FLAG_Tag-R
    • Myc Tag: Myc_Tag-F and Myc_Tag-R
    • HA Tag: HA_Tag-F and HA_Tag-R
    • GCN4 Leucine Zipper: GCN4-F and GCN4-R2
  • Same protocol as 1.21.2008