User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2017/01/05

From OpenWetWare
Jump to navigationJump to search
Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

Test nanoparticle reaction using N-acetylated (or methylated) amino acids.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock solutions

  1. N-acetyl serine
    1. Supplier: Carbosynth
    2. 22.72 mg (solid was gooey. could be that way or have taken on water. check later)
    3. to 10.0 mL water
    4. 147.13 g/mol
    5. 1.54 mM
  2. N-acetyl asparagine
    1. Supplier: ChemImpex
    2. 5.85 mg
    3. to 10.0 mL water
    4. 174.16 g/mol
    5. 3.35 mM
  3. N-acetyl isoleucine
    1. Supplier: ChemImpex
    2. 5.41 mg
    3. to 10.0 mL water
    4. 173.22 g/mol
    5. 3.12 mM
  4. N-methyl D,L aspartic acid hydrate
    1. Supplier: ChemImpex
    2. 4.81 mg
    3. to 10.0 mL water
    4. 165.1 g/mol
    5. 2.91 mM
  5. N-acetyl leucine
    1. Supplier: ChemImpex
    2. 4.09 mg
    3. to 10.0 mL water
    4. 173.2 g/mol
    5. 2.36 mM
  6. N-acetyl methionine
    1. Supplier: ChemImpex
    2. 2.6 mg
    3. to 10.0 mL water
    4. 191.29 g/mol
    5. 1.36 mM
  7. N-acetyl lysine
    1. Supplier: ChemImpex
    2. 2.86 mg
    3. to 10.0 mL water
    4. 188.2 g/mol
    5. 1.52 mM
  8. N-acetyl histidine hydrate
    1. Supplier: ChemImpex
    2. 3.98 mg
    3. to 10.0 mL water
    4. 215.2 g/mol
    5. 1.85 mM
  9. N-acetyl phenylalanine
    1. Supplier: ChemImpex
    2. 6.56 mg
    3. to 10.0 mL water
    4. 207.2 g/mol
    5. 3.17 mM
  10. N-acetyl threonine
    1. Supplier: ChemImpex
    2. 2.66 mg
    3. to 10.0 mL water
    4. 161.2 g/mol
    5. 1.65 mM
  11. N-acetyl proline
    1. Supplier: Alfa Aesar
    2. 5.29 mg
    3. to 10.0 mL water
    4. 157.81 g/mol
    5. 3.35 mM
  12. N-acetyl tryptophan
    1. Supplier: ChemImpex
    2. 7.74 mg
    3. to 10.0 mL water
    4. 246.3 g/mol
    5. 3.14 mM
  13. N-acetyl alanine
    1. Supplier: ChemImpex
    2. 7.1 mg
    3. to 10.0 mL water
    4. 131.1 g/mol
    5. 5.42 mM
  14. N-acetyl cysteine
    1. Supplier: ChemImpex
    2. 5.3 mg
    3. to 10.0 mL water
    4. 163.2 g/mol
    5. 3.24 mM
  15. N-acetyl tyrosine
    1. Supplier: ChemImpex
    2. 2.12 mg
    3. to 10.0 mL water
    4. 223.22 g/mol
    5. 0.950 mM
  16. N-acetyl glutamic acid
    1. Supplier: ChemImpex
    2. 2.7 mg
    3. to 10.0 mL water
    4. 189.17 g/mol
    5. 1.43 mM
  17. N-acetyl valine
    1. Supplier: ChemImpex
    2. 6.15 mg
    3. to 10.0 mL water
    4. 159.2 g/mol
    5. 3.86 mM
  18. N-acetyl arginine dihydrate
    1. Supplier: ChemImpex
    2. 5.28 mg
    3. to 10.0 mL water
    4. 252.27 g/mol
    5. 2.09 mM
  19. N-acetyl glutamine
    1. Supplier: ChemImpex
    2. 2.88 mg
    3. to 10.0 mL water
    4. 188.2 g/mol
    5. 1.53 mM
  20. N-acetyl glycine
    1. Supplier: ChemImpex
    2. 5.05 mg
    3. to 10.0 mL water
    4. 117.1 g/mol
    5. 4.31 mM
  21. AuCl3
    1. 13.93 mg
    2. to 10.0 mL water
    3. 303.3 g/mol
    4. 4.59 mM
  22. HCl
    1. 1 mM

Samples (100 uM amino acid, 250 uM gold, pH 4) (Volumes in uL)

  1. N-acetyl serine
    1. N-acetyl serine: 32.4
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4195
  2. N-acetyl asparagine
    1. N-acetyl asparagine: 148.9
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4079
  3. N-acetyl isoleucine
    1. N-acetyl isoleucine: 160.1
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4068
  4. N-methyl D,L Aspartic Acid
    1. N-methyl D,L Aspartic Acid: 171.6
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4056
  5. N-acetyl leucine
    1. N-acetyl leucine: 211.7
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4016
  6. N-acetyl methionine
    1. N-acetyl methionine: 367.9
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3860
  7. N-acetyl lysine
    1. N-acetyl lysine: 329.0
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3899
  8. N-acetyl histidine
    1. N-acetyl histidine: 270.4
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3957
  9. N-acetyl phenylalanine
    1. N-acetyl phenylalanine: 157.9
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4070
  10. N-acetyl threonine
    1. N-acetyl threonine: 303.0
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3925
  11. N-acetyl proline
    1. N-acetyl proline: 149.2
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4079
  12. N-acetyl tryptophan
    1. N-acetyl tryptophan: 159.1
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4069
  13. N-acetyl alanine
    1. N-acetyl alanine: 92.3
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4135
  14. N-acetyl cysteine
    1. N-acetyl cysteine: 154.0
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4074
  15. N-acetyl tyrosine
    1. N-acetyl tyrosine: 3701
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3701
  16. N-acetyl glutamic acid
    1. N-acetyl glutamic acid: 350.3
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3877
  17. N-acetyl valine
    1. N-acetyl valine: 129.4
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4098
  18. N-acetyl arginine
    1. N-acetyl arginine: 238.9
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3989
  19. N-acetyl glutamine
    1. N-acetyl glutamine: 326.7
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 3901
  20. N-acetyl glycine
    1. N-acetyl glycine: 115.9
    2. AuCl3: 272
    3. HCl: 500
    4. Water: 4112


Note: The methionine sample was tinged purple and the tryptophan was clearly purple before the samples went into the oven. All other samples were colorless, save for the yellowish gold color.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2017/01/05&oldid=1018850"