User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/29

Biomaterials Design Lab

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Objective Calculations for stock solutions to be made tomorrow. Description stock solutions 1 L of 0.001 M HCl Concentrated HCl should be: 11.65 M M1V1 = M2V2 (11.65 M)(V1) = (0.001M)(1L) V1 = 85.8 μL 1 L of 0.001 M HNO3 Concentrated HNO3 should be: 15.8 M M1V1 = M2V2 (15.8 M)(V1) = (0.001M)(1L) V1 = 63.2 μL 1 L of 1 M NaOH MW of NaOH = 39.997 g/mol M = (m/MW)/V 1 M = (m/39.997 g/mol)/1L m = 39.997 g 1 L of 0.001 M NaOH Use 1M stock solution M1V1 = M2V2 (1 M)(V1) = (0.001M)(1L) V1 = 1 mL 10 mL of 12.5 mM Fructose MW = 180.16 g/mol 0.0125 M = (m/180.16 g/mol)/(0.010 L) m = 22.5 mg 10 mL of 2.5 mM AuCl3 MW = 303.33 g/mol 0.0025 M = (m/303.33 g/mol)/(0.010 L) m = 7.58 mg Protein (BSA) MW = 66776 g/mol 30 μM = (m/66776 g/mol)/(0.010 L) m = 20.0 mg sample solutions 0.25 mM AuCl3, 0 mM fructose, pH 4 ([H+] = 1x10-4 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of HCl stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-4 M)(5 mL) VHCl stock = 0.5 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.483 mL 0.25 mM AuCl3, 0 mM fructose, pH 5 ([H+] = 1x10-5 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of HCl stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-5 M)(5 mL) VHCl stock = 0.05 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.933 mL 0.25 mM AuCl3, 0 mM fructose, pH 6 ([H+] = 1x10-6 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of HCl stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-6 M)(5 mL) VHCl stock = 0.005 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.978 mL 0.25 mM AuCl3, 0 mM fructose, pH 7 ([H+] = 1x10-7 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of HCl stock VHCl stock = 0 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.983 mL 0.25 mM AuCl3, 0 mM fructose, pH 8 ([H+] = 1x10-8 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of 0.001 M NaOH stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-6 M)(5 mL) VNaOH stock = 0.005 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.978 mL 0.25 mM AuCl3, 0 mM fructose, pH 9 ([H+] = 1x10-9 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of 0.001 M NaOH stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-5 M)(5 mL) VNaOH stock = 0.05 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.933 mL 0.25 mM AuCl3, 0 mM fructose, pH 10 ([H+] = 1x10-10 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of 0.001 M NaOH stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-4 M)(5 mL) VNaOH stock = 0.5 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.483 mL 0.25 mM AuCl3, 0 mM fructose, pH 11 ([H+] = 1x10-11 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of 1 M NaOH stock M1V1 = M2V2 (1 M)V1 = (1x10-3 M)(5 mL) VNaOH stock = 0.005 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.978 mL 0.25 mM AuCl3, 0 mM fructose, pH 12 ([H+] = 1x10-12 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock Vfructose stock = 0 mL Volume of 1 M NaOH stock M1V1 = M2V2 (1 M)V1 = (1x10-2 M)(5 mL) VNaOH stock = 0.05 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.933 mL 0.25 mM AuCl3, 0 mM fructose, pH 4 ([H+] = 1x10-4 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of HCl stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-4 M)(5 mL) VHCl stock = 0.5 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.458 mL 0.25 mM AuCl3, 0 mM fructose, pH 5 ([H+] = 1x10-5 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of HCl stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-5 M)(5 mL) VHCl stock = 0.05 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.908 mL 0.25 mM AuCl3, 0 mM fructose, pH 6 ([H+] = 1x10-6 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of HCl stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-6 M)(5 mL) VHCl stock = 0.005 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.953 mL 0.25 mM AuCl3, 0 mM fructose, pH 7 ([H+] = 1x10-7 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of HCl stock VHCl stock = 0 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock Vwater = 3.958 mL 0.25 mM AuCl3, 0 mM fructose, pH 8 ([H+] = 1x10-8 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of 0.001 M NaOH stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-6 M)(5 mL) VNaOH stock = 0.005 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.953 mL 0.25 mM AuCl3, 0 mM fructose, pH 9 ([H+] = 1x10-9 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of 0.001 M NaOH stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-5 M)(5 mL) VNaOH stock = 0.05 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.908 mL 0.25 mM AuCl3, 0 mM fructose, pH 10 ([H+] = 1x10-10 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of 0.001 M NaOH stock M1V1 = M2V2 (1x10-3 M)V1 = (1x10-4 M)(5 mL) VNaOH stock = 0.5 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.458 mL 0.25 mM AuCl3, 0 mM fructose, pH 11 ([H+] = 1x10-11 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of 1 M NaOH stock M1V1 = M2V2 (1 M)V1 = (1x10-3 M)(5 mL) VNaOH stock = 0.005 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.953 mL 0.25 mM AuCl3, 0 mM fructose, pH 12 ([H+] = 1x10-12 M), 5 mL total Volume of AuCl3 stock M1V1 = M2V2 (0.0025 M)V1 = (0.00025 M)(5 mL) VAuCl3 stock = 0.5 mL Volume of fructose stock M1V1 = M2V2 (0.0125 M)V1 = (0.0000625 M)(5 mL) Vfructose stock = 0.025 mL Volume of 1 M NaOH stock M1V1 = M2V2 (1 M)V1 = (1x10-2 M)(5 mL) VNaOH stock = 0.05 mL Volume of Protein stock M1V1 = M2V2 (30 μM)VProtein Stock = (3.1 μM))(5 mL) VProtein Stock = 0.517 mL Volume of water Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock Vwater = 3.908 mL Data Add data and results here... 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