User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/25

WiFi coli: A communicolight system	Main project page Previous entry      Next entry
By the way... Today, I left the ligation for the mutated red firefly luciferases to insert into the dephosphated J61002 vector containing the J23102 constitutive promoter. -H2O --------------------> 7μL -Buffer ligase ----------> 4μL -Vector (plasmid) -----> 2μL -Insert (luciferase) ---> 6μL -Ligase -----------------> 1μL