User:Maria Briscione/Notebook/Chem 571/2012/02/08

Biomaterials Design Lab

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Objective Fluorescently label cysteine residues on BSA Description Procedure 406 microliters of the phosphate buffer (pH 7.04) 2.9 mg of 5-iodoacetamido flourescin 2.776 mL BSA from Stock BSA concentration - 13.086 µM let reaction sit for at least two hours in dialysis bag dialysis over a week, water was changed daily Data Notes Ideal ph is between 7 and 8 deprotonates at this pH labeling was completed in order to determine binding of the cysteine to the dye this procedure also allows us to determine concentration