User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/13

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November 13, 2013 Procedures Synthesis of ZnPPIX 1:1 molar ratio of Zinc nitrate hexahydrate to Dr Miller's porphyrin Dissolve 0.0054g Zn nitrate and 0.0144g porphyrin in 5mL DMF Take absorbance measurements every 20 minutes, making 100x dilutions Continue heating until absorbance spectra stops changing (80 minutes) Data <br.> Beer's Law calculations: Notes In the third, fourth, and fifth samples, it is evident that the spectra stopped changing and the reaction was assumed to be complete