July 8, 2013
Notes from Meeting
- Since we're seeing some noise from just PBS on the FCS: make new PBS and run, parse out 10mL samples to avoid contaimination
- Sonicate or centrifuge green fluorescent beads before running to prevent aggregation while measurements are being taken
- Go up to 500pM for Oligo D samples (and lower concentrations)
- Use a high, constant concentration of DNA with lower, decreasing concentration of MB
- Check for consistent diffusion times between samples run on different days
- Different diffusion times indicate background is something other than unbound DNA
- Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples
- Look in literature for ways to calculate silica shell thickness without TEM results
FCS Sample Prep
- Green fluorescent beads
- 10000x diluted
- Run 3x, 90s
- Oligo D
- 150pM
- 125pM
- 100pM
- Run 3x, 500s
- DNA/MB: DNA held at a constant concentration to isolate background from DNA
- 600/500
- 600/400
- 600/300
- 600/200
- 600/100
- Run 3x, 500s
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