User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/02
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July 2, 2013FCS Sample Prep
FCS Data<br.> Beads used to align laser; three samples run at 90s each, average values used. Curve turned out as expected.<br.> <br.> Oligo D: three samples run at each concentration, 500s each, average values used. Y-intercepts increase as concentration decreases, as expected.<br.> <br.> DNA/MB: three samples run at each concentration, 500s each, average values used. Y-intercepts increase as concentration decreases until 50pM. DNA in excess rather than MB to ensure binding.<br.> <br.> Calibration graphs for Oligo D and DNA/MB samples. Oligo D trend turned out as expected; DNA/MB trend dropped at 50pM concentration. DNA/MB samples will be remade and run again tomorrow. <br.> |