User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/12

Main project page Previous entry      Next entry

June 12, 2013 Finished MHA silica-coated AuNPs, resuspended in water, 10x and 100x dilutions made for all four samples Fluorescent beads and Alexa 532 antibodies successfully run and analyzed on FCS Green fluorescent beads, diluted 10000x Alexa 532 antibodies, diluted to 500pM and 100pM Notes Take absorbance spectra of sonicated original AuNP dilutions Take absorbance spectra of 10x and 100x silica-coated AuNP dilutions Take fluorescence spectra of ZnPPIX/AuNP, DNA/ThT combinations Add ZnPPIX/AuNP fluorescence data to graph for presentation Make DNA/ThT calibration curve (with fluorescence data)