User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/15

Experimental Biological Chemistry: Fall 2013

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October 15, 2013 Objective Make four samples of HRP-AuNPs. Description Au:HRP Ratios (with corresponding concentrations and volumes to make 10mL)<br.> 30:1 - 0.25mM:8.33uM - 850uL:5.11mL, diluted up to 10mL with distilled water in a volumetric flask 60:1 - 0.25mM:4.17uM - 850uL:2.56mL, diluted up to 10mL with distilled water in a volumetric flask 90:1 - 0.25mM:2.78uM - 850uL:1.71mL, diluted up to 10mL with distilled water in a volumetric flask 120:1 - 0.25mM:2.08uM - 850uL:1.28mL, diluted up to 10mL with distilled water in a volumetric flask Leave samples in 80C oven for three hours.