User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/21

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Purpose

  • DAY 5 - Continue running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.

Procedure

  • Three flasks were taken out of the drawer: one flask containing silver-incorporated film, one containing control film, and one containing no film.
  • Starter pellet was taken out of freezer and defrosted in room temperature.
  • 3mL of LB were added to starter pellet and cells were resuspended.
  • 1mL of each resuspended cell culture was added into each flasks.
  • All flasks were placed in incubator shaker at 230rpm, 37°C, for 15 hours.

Observation

  • The flasks containing the control films only appeared cloudy, indicating the flasks were contaminated.


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