User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/12

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12/12/12 Cultures (KAH111/C-His): Pick colonies, grow overnight Cultures KAH111/C-His assembly from 12/11/12 Picked 4 colonies at 11 am; saw no growth at 6:30 pm Picked 4 more colonies; incubated at 37°C with shaking overnight Culture = 2mL LB Amp (100 μg/mL)