User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01

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12/01/12 Cultures/ minipreps: KAH3/MV2 colonies (2) Minipreps Check with PstI digest Compare to empty vector MV2 Reagent Volume Expected: 1. MV2 = 4529 2. KAH3/MV2-1 = 5280 3. KAH3/MV2-2 = 5280 15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmid. Ladder DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 9.5   15.0 μL 37°C/ ~15 min.