User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/06

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07/06/11 Cultures/minipreps: KAH183-177/MV8 (4) Sequencing order: KAH183-177/MV8 (4), and Daniel's miR-344 sensors Minipreps > Check via sequencing --> Primers: f = DB122, r = BGHR KH11, 183-177MV8-1, f: 7/7/11 no priming KH11, 183-177MV8-1, r: 7/7/11 no priming KH11, 183-177MV8-2, f: 7/7/11 smear; 7/8/11 re-run, good KH11, 183-177MV8-2, r: 7/7/11 smear; 7/8/11 re-run, good KH11, 183-177MV8-3, f: 7/7/11 poor quality KH11, 183-177MV8-3, r: 7/7/11 no priming KH11, 183-177MV8-4, f: 7/7/11 smear; 7/8/11 re-run, good KH11, 183-177MV8-4, r: 7/7/11 smear; 7/8/11 re-run, good