User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/27

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Objectives

Plasmid extraction

  • Performed with LB-liquid mediums from yesterday.
  • I used Miguel´s Lab miniprep.
  • Final dilution in 50μL

Restriction

  • In order to standardize pBBRmc-S5 I will cut PCR Purification with SacI & ApaI.
Reactives Dilutions
Buffer4 10x NEB 2μL
BSA 1μL
PCR Purf. 12μL
H2O 4 μL
ApaI 1μL
SacI 1μL


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