Friday 18th June 2010
Today I performed 2 double restrictions:
- GFP cut with XbaI and PstI
- Plasmid 18 cut with EcoRI and PstI
as follows:
For GFP
Reactive...........1x [ul]
DNA................25
BSA..................1
Buffer2............4
H2O................7
PstI................1.5
XbaI...............1.5
Total...............40
Reactive...............1x [ul]
DNA....................10
BSA......................1
Buffer2................3
H2O...................13
EcoRI-HF.............1.5
PstI......................1.5
Total..................30
I left the digestions in the incubator overnight.
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