User:Jarle Pahr/Strains

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Notes on various bacterial strains:

See also http://openwetware.org/wiki/Strains

E. coli:


Strain Description Heritage References Comments
Dh5a
BW21342
BW25083
BW25113 [BW25113 at Ecoliwiki Parent strain of the Keio single-deletion mutant collection.
BW21578rrnBT14 ΔlacZWJ16BD792 via BW21342
BW22773lacIq rrnBT14 ΔlacZWJ16 proC::Tn5-132;
BW26434 (CGSC #7658)Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514
BW27784 Expresses arabinose transporter constitutively.
BW22786 Expresses arabinose transporter constitutively.
MG1655K-12 derivate  ?Starves for pyrimidine in minimal medium: http://www.ncbi.nlm.nih.gov/pubmed/8501045


Note on LacI satus of BW strains (copied from http://openwetware.org/wiki/E._coli_genotypes):

"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113.

As strains BW27784 and BW22786 were also constructed from BW25113, the former should also be LacI+.


Nomenclature:

Insertion mutation.


Bibliography:


Khlebnikov 2001: http://mic.sgmjournals.org/content/147/12/3241.long


http://web.mit.edu/7.02/pdfs/nomenclature.pdf


CGSC - Coli Genetic Stock Center: http://cgsc.biology.yale.edu/index.php


http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp

Verification

Mutation-specific primers for verification of strains by PCR:

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