User:Jarle Pahr/Strains
Notes on various bacterial strains:
See also http://openwetware.org/wiki/Strains
E. coli:
Strain | Description | Heritage | References | Comments |
---|---|---|---|---|
Dh5a | ||||
BW21342 | ||||
BW25083 | ||||
BW25113 | [BW25113 at Ecoliwiki | Parent strain of the Keio single-deletion mutant collection. | ||
BW21578 | rrnBT14 ΔlacZWJ16 | BD792 via BW21342 | ||
BW22773 | lacIq rrnBT14 ΔlacZWJ16 proC::Tn5-132; | |||
BW26434 (CGSC #7658) | Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514 | |||
BW27784 | Expresses arabinose transporter constitutively. | |||
BW22786 | Expresses arabinose transporter constitutively. | |||
MG1655 | K-12 derivate | ? | Starves for pyrimidine in minimal medium: http://www.ncbi.nlm.nih.gov/pubmed/8501045 |
Note on LacI satus of BW strains (copied from http://openwetware.org/wiki/E._coli_genotypes):
"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113.
As strains BW27784 and BW22786 were also constructed from BW25113, the former should also be LacI+.
Nomenclature:
- Insertion mutation.
Bibliography:
Khlebnikov 2001: http://mic.sgmjournals.org/content/147/12/3241.long
http://web.mit.edu/7.02/pdfs/nomenclature.pdf
CGSC - Coli Genetic Stock Center: http://cgsc.biology.yale.edu/index.php
http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp
Verification
Mutation-specific primers for verification of strains by PCR: