Notes on various bacterial strains:
See also http://openwetware.org/wiki/Strains
|BW25113||[BW25113 at Ecoliwiki||Parent strain of the Keio single-deletion mutant collection.|
|BW21578||rrnBT14 ΔlacZWJ16||BD792 via BW21342|
|BW22773||lacIq rrnBT14 ΔlacZWJ16 proC::Tn5-132;|
|BW26434 (CGSC #7658)||Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514|
|BW27784||Expresses arabinose transporter constitutively.|
|BW22786||Expresses arabinose transporter constitutively.|
|MG1655||K-12 derivate||?||Starves for pyrimidine in minimal medium: http://www.ncbi.nlm.nih.gov/pubmed/8501045|
Note on LacI satus of BW strains (copied from http://openwetware.org/wiki/E._coli_genotypes):
"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113.
As strains BW27784 and BW22786 were also constructed from BW25113, the former should also be LacI+.
- Insertion mutation.
Khlebnikov 2001: http://mic.sgmjournals.org/content/147/12/3241.long
CGSC - Coli Genetic Stock Center: http://cgsc.biology.yale.edu/index.php
Mutation-specific primers for verification of strains by PCR: