User:Jarle Pahr/DNA

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Structure:

http://www.chemguide.co.uk/organicprops/aminoacids/dna1.html


Analysis and quantification http://nucleicacids.bitesizebio.com/articles/how-to-identify-supercoils-nicks-and-circles-in-plasmid-preps/

Quick reference - Determining DNA concentration and purity: http://nucleicacids.bitesizebio.com/articles/dna-concentration-purity/

http://www.pathologyoutlines.com/topic/moleculardnapuranalyzingpurity.html

http://nanodrop.com/Library/CPMB-1st.pdf

http://eu.idtdna.com/pages/decoded/decoded-articles/pipet-tips/decoded/2013/01/18/oligo-quantification-getting-it-right?utm_source=Twitter&utm_medium=Tweet&utm_campaign=decoded%2B3.1

Preticipation:

http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=544&criteria=


Isolation:

DNA isolation methods anno 1999: http://rothlab.ucdavis.edu/protocols/DNA-from-Agarose-Review.html

Gel extraction by glass wool filtering: http://www.jcancer.org/v03p0093.htm

http://nucleicacids.bitesizebio.com/articles/10-tips-for-better-dna-gel-extraction-results/

http://nucleicacids.bitesizebio.com/articles/5-more-tips-for-dna-gel-extraction/

How alkalyine lysis works: http://bitesizebio.com/articles/the-basics-how-alkaline-lysis-works/

How to get better midiprep yields: http://nucleicacids.bitesizebio.com/articles/how-to-get-better-plasmid-midiprep-yields/

A menagerie of miniprep methods: http://bitesizebio.com/articles/a-menagerie-of-mini-prep-methods/

http://www.ncbi.nlm.nih.gov/pubmed/388356

A One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023457

http://nucleicacids.bitesizebio.com/articles/how-to-maximize-the-quantity-and-quality-of-purified-plasmids/

http://nucleicacids.bitesizebio.com/articles/better-plasmid-midipreps-part-ii-what-causes-low-yields/


http://nucleicacids.bitesizebio.com/articles/tech-clinic-3-dna-digestion-precipitation-and-clean-up/

http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/manip/conc.html

http://bioinfoweb.com/Protocol-plasmid-DNA-isolation-by-alkaline-lysis-method-miniprep.htm

http://www.mobio.com/blog/2013/03/27/where-oh-where-did-my-dna-go-a-post-on-dna-cleanup/


Comparison of alkaline-lysis protocols:


Gel purification:

Centrifugal filtration: http://www.zenbiology.com/2012/03/06/dna-extraction-from-agarose-gels-centrifugal-filtration/

http://www.ncbi.nlm.nih.gov/pubmed/10094803

Physical data:

http://www.nordicbiosite.com/viewpage.aspx?pageid=33

http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/rna-tools-and-calculators/dna-and-rna-molecular-weights-and-conversions.html


Amplification:


PCR:


LAMP:

http://nar.oxfordjournals.org/content/28/12/e63.full


MLPA: http://en.wikipedia.org/wiki/Multiplex_ligation-dependent_probe_amplification

http://www.nito.no/Fagmiljoer/Bioingeniorfaglig-institutt/Bioingenioren/Alle-Bioingeniorene/Bioingenioren-2006/Bioingenioren-12-2006/Utproving-av-molekylaranalyse-av-mikrodelesjoner-ved-hjelp-av-MLPA/


Misc.:


http://www.ehow.com/how_8554213_evaporate-dna-solution.html


DNA elution from paper:

http://wiki.answers.com/Q/How_do_you_elute_DNA_from_filter_paper

http://2011.igem.org/Team:Cambridge/Protocols/Filter_Paper#/Protocols/Filter_Paper

http://www.bio.net/bionet/mm/methods/2003-June/096480.html

http://www.scribd.com/doc/23031907/Instructions-for-Elution-of-Plasmids-From-Whatman-Paper-2008

http://www.scientistsolutions.com/t10099-extraction+of+plasmid+dna+from+the+paper+.html

http://www.protocol-online.org/biology-forums/posts/10829.html


Storage and stability:


DNA origami:

http://cadnano.org/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731887/

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