Thoughts on characterisation of strains, plasmids and combinations thereof:
Types of characterisation that can be performed:
Maximal cell density.
For fluorescent reporter constructs:
Note that depending on the stability of the fluorescent, fluorescence may correlate with recent gene activity, or total (accumulated) gene activity over the lifetime of the culture.
Fluorescence/gene expression displayed by saturated cultures.
Fluorescence displayed by exponentially growing cultures.
Fluorescence/gene expression displayed in different media.
Fluorescence/gene expression displayed for different incubation conditions (temperature, incubation in plate reader vs. shake flask, etc.)
The Spinach RNA aptamer as a characterisation tool for synthetic biology: http://pubs.acs.org/doi/abs/10.1021/sb400089c