User:Jarle Pahr/Buffers

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Qiagen EB buffer: 10 mM Tris-HCl, pH 8.5.

See also http://people.mbi.ucla.edu/sumchan/qiagenbuffer.html


Buffer Composition Use Storage Hazardous chemicals? Comments
TAE40mM Tris, 20mM acetic acid, and 1mM EDTAElectrophoresisRoom temperature
Qiagen EB 10 mM Tris-HCl, pH 8.5 DNA elution and storage Room temperature -
TE (Tris-EDTA) 10 mM Tris-HCl, pH 8.5 + 10 mM Tris-HCl, pH 8 + 1 mM EDTA DNA elution and storage  ? - EDTA inhbitis nucleases and also inhibits sequencing reactions.
NEBuffer 1 10mM Bis-Tris-Propane-HCl, 10mM MgCl2,1mM DTT,pH 7.0@25°C Restriction digestion-20C - CIP shows 50 % activity in NEBuffer 1, compared with 100 % activity in NEBuffers 2-4.
NEBuffer 250mM NaCl,10mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°CRestriction digestion-20C -
NEBuffer 3100mM NaCl,50mM Tris-HCl,10mM MgCl2,1mM DTT,pH 7.9@25°CRestriction digestion-20C -
NEBuffer 450mM Potassium Acetate,20mM Tris-acetate,10mM Magnesium Acetate,1mM DTT,pH 7.9@25°CRestriction digestion-20C -
Phosphate Buffered Saline (PBS) 8 g/L NaCl, 0.2 g/L KCl, 1.44 g/L Na2HPO4, 0.22 g/L KH2PO4. Adjust pH to 7.4 with HCl.http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8247.full?text_only=true

Transformation and Storage Solution (TSS):

LB with 10 wt/vol % polyethylene glycol (PEG), 5 % v/v DMSO, 50 mM Mg2+, pH 6.5.


HEPES:

http://en.wikipedia.org/wiki/HEPES

MOPS:

http://en.wikipedia.org/wiki/MOPS

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