User:Isis Trenchard/Notebook/BioE44 Stuff/2010/03/27

Project name

finish cloning
- 002, 003, 004 into no promotor plasmids
- transform into E. coli.
overnight cultures
sterile water
aspirators need to be set up (for first day actually..)
add ethanol to PE
aliquot miniprep buffers (into 1.5mL centrifuge tubes)
- P1 - 250uL
- P2 - 250uL
- N3 - 350uL
- PE - 750uL
restriction enzymes... dilute before class? aliquot?

Stuff to make for M1:D1