User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/02
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BBa_I13504+BBa_katEThe following steps were performed towards a new composited part Check overnight growth (BBa_I13504)Growth worked fine - all three tubes grew. Take Glycerol Stock (BBa_I13504)For each tubes make glycerol stock
Miniprep samples(BBa_I13504)Purify plasmids using QIAGen Miniprep kit
Digestion4 Digestions where setup
GelThe digestion was run on a 2% Gel. Check against BBa_I13504 sample
5x M9 Salt, 200mlMinimal media M9 salt, at pH7
Autoclave at 121ºC for 15 minutes M9 Media, 200ml
Carbon Sources
5 bottles, 10ml 50% Glycerol
Autoclave 20min in liquid cycle Effector
0.1M CaCl2, 500mlUsed to prepare competent cells. CaClx6H2O has a molecular weight of FW 219.08.
Autoclave at 121ºC for 15minutes 0.1M MgCl2, 500mlUsed to prepare competent cells. MgCl2x6H2O has a molecular weight of FW 203.3.
Autoclave at 121ºC for 15minutes 2x 500ml waterAutoclave at 121ºC for 15minutes Locate Potassium L-lactateI am currently missing this substrate to grow my nanomagentic bacteria. Test FSM with Pyruvic as Carbon SourceSince I have not ben able to locate lactate I am testing pryuvic acid as a carbon source. To 200ml FSM I added 0.47g powder
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