User:Hetmann/Notebook/2006-11-20
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Notes
hetmann, im confused - found the 4 tubes and nanodropped them (theyre all above 100, yay!) but I have no idea what the colors mean...
- The tubes are color coded to match the stickers on the beakers found on Desk 1 of Room 5096.
- Yellow: 104.5
- Blue: 122.3
- Green:137.7
- Pink: 161.7
Put back in freezer. LMK what the colors are and ill do the digests required for the ligations you put on 11/18.
Digestion
Doing the digests, 8 samples ea.
J36335 /sp:
- 7.6 uL DNA (64.6)
- 1 uL 10x BSA (8.5)
- 1 uL NEBuffer 2 (8.5)
- 0.2 uL SpeI (1.7)
- 0.2 uL PstI (1.7)
B0015 /sp:
- 7.6 uL DNA
- 1 uL 10x BSA
- 1 uL NEBuffer 2
- 0.2 uL SpeI
- 0.2 uL PstI
J36832 /xp:
- 7.6 uL DNA
- 1 uL 10x BSA
- 1 uL NEBuffer 3
- 0.2 uL XbaI
- 0.2 uL PstI
J36834 /xp:
- 7.6 uL DNA
- 1 uL 10x BSA
- 1 uL NEBuffer 3
- 0.2 uL XbaI
- 0.2 uL PstI
12h@45, 20min@80. in PCR machine in small room. -ZS
labeling
relabeled all the pre-digested plasmids with new labeling convention; put new labeling convention on fridge.
Goals for 11/18
- Progression
Step 1: Midiprep- B0015
- J04500+KaiB eg.
- J04500+KaiC
- J04500+KaiA+J04500+KaiC
Step 2: Digestion- B0015/SP
- J04500+KaiB/XP (J36832)
- J04500+KaiC/XP (J36834)
J04500+KaiA+J04500+KaiC/SP (J36335)
- Progression