User:Hetmann/Notebook/2006-11-20

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Contents

Notes

hetmann, im confused - found the 4 tubes and nanodropped them (theyre all above 100, yay!) but I have no idea what the colors mean...

  • The tubes are color coded to match the stickers on the beakers found on Desk 1 of Room 5096.
  • Yellow: 104.5
  • Blue: 122.3
  • Green:137.7
  • Pink: 161.7

Put back in freezer. LMK what the colors are and ill do the digests required for the ligations you put on 11/18.

Digestion

Doing the digests, 8 samples ea.

J36335 /sp:

  • 7.6 uL DNA (64.6)
  • 1 uL 10x BSA (8.5)
  • 1 uL NEBuffer 2 (8.5)
  • 0.2 uL SpeI (1.7)
  • 0.2 uL PstI (1.7)

B0015 /sp:

  • 7.6 uL DNA
  • 1 uL 10x BSA
  • 1 uL NEBuffer 2
  • 0.2 uL SpeI
  • 0.2 uL PstI

J36832 /xp:

  • 7.6 uL DNA
  • 1 uL 10x BSA
  • 1 uL NEBuffer 3
  • 0.2 uL XbaI
  • 0.2 uL PstI

J36834 /xp:

  • 7.6 uL DNA
  • 1 uL 10x BSA
  • 1 uL NEBuffer 3
  • 0.2 uL XbaI
  • 0.2 uL PstI

12h@45, 20min@80. in PCR machine in small room. -ZS

labeling

relabeled all the pre-digested plasmids with new labeling convention; put new labeling convention on fridge.

Goals for 11/18

    • Progression
      • Step 1: Midiprep
        1. B0015
        2. J04500+KaiB eg.
        3. J04500+KaiC
        4. J04500+KaiA+J04500+KaiC
      • Step 2: Digestion
        1. B0015/SP
        2. J04500+KaiB/XP (J36832)
        3. J04500+KaiC/XP (J36834)
        4. J04500+KaiA+J04500+KaiC/SP (J36335)
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