User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29
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29 May 2013
-conc. from 10mL LB to 1.5mL -blinded for adam: L4440=squiggles, GFP=wiggles
Per 1 L of M9: Adjusted for 700mL Notes: -KH2PO4 3g 2.1g FisherChemicals F.W.141.96, lot no. 005502 Date:8.07.00 -Na2HPO4 6g 4.2g FisherChemicals F.W. 136.09, lot no. 995072 Date? -NaCl 5g 3.5g NaCl F.W. 58.44 -H2O to 1L water to 700mL MilliQ, prepared by Skyler -read below 700µL MgSO4 1 M prepared by Lena, aliquoted 29 May 2013 Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave) add 1mL of MgSO4 sterily
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