User:Guido/Notes/July 07

July 3, 2007

• Do a culture pcr from last recombination of specR to replace MutS

• • 3 μl culture
  • 1 μl f-primer
  • 1 μl r-primer
  • 45 μl supermix
  • 50 μl reaction

* 95  10 min

* 95  30 sec  
* 51  30 sec
* 72  2 min
* X30

* 72   7 min
* 4 forever