User:Guido/Notes/July 07

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July 3, 2007

  • Do a culture pcr from last recombination of specR to replace MutS


    • 3 μl culture
    • 1 μl f-primer
    • 1 μl r-primer
    • 45 μl supermix
    • 50 μl reaction


* 95  10 min

* 95  30 sec  
* 51  30 sec
* 72  2 min
* X30

* 72   7 min
* 4 forever
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