User:Fasih M. Ahsan/Notebook/MIMG 199 W15/2015/01/20
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Tamura Sequence
1. Added 6.25g of Difco LB and 3.75g of Bacto Agar to 250mL DI Water. Autoclaved at 121 degree Celsius. Added 250 uL 1000x Ampicillin (100 mg/mL) at 55 degrees Celsius to media and swirled. Poured approximately 25mL per plate. Allowed to harden, inverted.
1. Plated on 6 LB Agar Amp plates each of the Tokyo G-Block glycerol stocks using glass pasteur pipets. Incubated at 37 degrees Celsius.
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