User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15

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Effect of Tris Buffer on Protein Fibers Main project page
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Objective

To synthesize a solution of AuNP, to continue to take UV-vis data over time of 70 Au/BSA solution synthesized on 01/31/11, and to determine the effect of tris buffer on protein fibers also synthesized on 01/31/11.

Description

Synthesizing AuNP

  1. Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube.
  2. Dilute to 20 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3 hr.
  5. Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on 01/31/12 over time.


Determining the effect of tris buffer on 166 Au/BSA ratio solution

  1. Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12.
  2. Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution).
  3. Allow fibers to settle.
  4. Take UV-vis data of tris/fiber solution.

Data

  • Graph of absorbance vs wavelength of the AuNP solution synthesized on 01/31/12 over time.
Image:Absorbance vs wavelength magnified 2-15-12.jpg


  • Graph of the peak absorbance value versus time of the AuNP solution synthesized on 01/31/12.
Image:Peak absorbance vs time 2-15-12.jpg

Over time, absorbance values increase dramatically.


  • Graph of the wavelength at which the peak absorbance was recorded versus time.
Image:Peak wavelength vs time 2-15-12.jpg

Over time, the optimum wavelength tends to get shorter.


  • Graph of absorbance vs wavelength of the protein fibers with added 50 mM tris buffer (pH 10.1)
Image:Absorbance vs wavelength tris 2-15-12.jpg


  • AuNP solution:
Image:Aunp solution.JPG

Notes



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