Objective
To try to control the aggregation process of AuNP/BSA.
Description
- Make 4 solutions of AuNP:
- Solutions 1 and 2 (Au/BSA mole ratio = 35)
- Combine 0.5 mL of 2.5 mM HAuCl4 and 0.0706 mL of 0.0177 mM BSA in a test tube.
- Add 5 mL of water.
- Place in oven at 70°C.
- Solutions 3 and 4 (Au/BSA mole ratio = 100)
- Combine 0.5 mL of 2.5 mM HAuCl4 and 2.02 mL of 0.0177 mM BSA in a test tube.
- Add 5 mL of water.
- Place in oven at 70°C.
- Leave solutions 1 and 3 in oven for 3.5 hr.
- Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.
Data
Solution 1: Au/BSA = 35, left in the oven
Solution 2: Au/BSA = 35, taken out of the oven every 30 minutes
Solution 3: Au/BSA = 100, left in the oven
Solution 4: Au/BSA = 100, taken out of the oven every 30 minutes
Notes
Solution 1 began to turn purple after 2 hr.
|