User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/08/24

Project name	Main project page Previous entry      Next entry
High Stability Plasmid Development Abstract pSB1AK3/B0015 digestion PCR digestion This is the second digestion reaction to link repC with the translational terminator B0015. The standard plasmid containing B0015 was digested with EcoRI-HF and XbaI: pSB1AK3/B0015 17μL BSA 1μL Buffer 4 5μL EcoRI-HF 1μL XbaI 1μL H2O mQ 25μL Total 50μL Inactivations will be done tomorrow.