User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/08/22
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High Stability Plasmid DevelopmentAbstractTransformation of pSB1T3/B0015 ligation TransformationI performed the transformation of the three ligations of the pSB1T3 plasmid (which confers resistance to Tetracyclin Tc) and the part B0015 (which confers resistance to Kanamicin Km) at ratios 1:50, 1:20 and 1:10. I used 40μL of competent cells and 2μL of the ligation reaction. I used our protocol. I will check the plated bacte4ria tomorrow and asses their growth. Petri dishes containes 75μL Km and 75μL Tc, resulting in a final concentration of 0.5μg/mL. |