User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/10/15

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Tasklist

  • SDS-PAGE
    • Warm your samples to about 40 °C using heating block
    • Transfer solutions to a premade gel (2 groups should share a gel...I have 4 - 5 samples as well)
    • Don't forget to use the premade protein ladder
    • Run as previously described
  • Dialysis
    • Be sure to measure protein in solution using Bradford(don't contaminate with the precipitated protein)
    • Set up for next week
    • measure UV & fluorescence of your original colloid solution (run water first to ensure prior protein has been removed)



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