User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/10/13

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SDS-PAGE Sample Buffer 2X recipe: 0.125 M Tris (pH adjusted to 6.8), 4% SDS, 20% glycerol, 0.01% bromophenol blue, & 5% 2-ME or 0.15 M DTT For 10 mL, no disulfide bond breaker 0.151 g Tris 0.40 g SDS 2 g glycerol 0.001 g bromophenol blue 7.5 mL H2O - mL HCl required to make pH 6.8 Actual prepared sample 7 mL H2O 0.151 g Tris ~2 mL 0.5 N HCl (ran out of 1 M HCl) -- pH 6.7 0.40 g SDS 0.001 g bromophenol blue (no DMSO available, but dissolved quickly this time) 2 g glycerol (added last) stored in 15 mL falcon tube (~11 mL) Unknown Protein Samples Soy protein 1 mg in 1.5 mL H2O (centrifuge vial) did not completely dissolve, even with 5 min vortex after settling, supernatant stored BSA 0.1 - 0.2 mg in 1 mL H2O (centrifuge vial) quickly dissolved - clear, colorless solution Casein 2.6 mg in 10 mL H2O (falcon tube) did not dissolve after 5 min vortex -- left in vial Horseradish Peroxidase (HRP) 0.4 mg in 3 mL H2O (falcon tube) quickly dissolved - clear, colorless solution