User:Dominique Brager/Notebook/481 Notebook/2017/09/05

Biomaterials Design Lab

Main project page Previous entry      Next entry

Objective Remake solutions with low Au:amino acid ratios over different pH range Use the following volumetric ratios of Au:AA:NaOH (1) 1:1:0.5 (2) 1:1:1 Switch to the 1 M NaOH solution and prepare mixtures using the following volumetric ratios (3) 1:1:0.1 (4) 1:1:1 Calculate theoretical pH assuming [AA] has negligible effect Titration Stock Solution Prep Tritration Standardization Description Theoretical pH Calculations Mol of HAuCl4 (2.5 mM) 1 mL* 1 L/1000 mL * 2.5 mmol/1 L * 1 mol/1000 mmol = 2.5*10^-6 mol HAuCl4 Mol of NaOH (5 mM and 1 M stock) 0.5 mL * 1 L/1000 mL * 5 mmol/1 L * 1 mol/1000 mmol = 2.5*10^-6 mol NaOH 1 mL * 1 L/1000 mL * 5 mmol/1 L * 1 mol/1000 mmol = 5*10^-6 mol NaOH 0.1 mL * 1 L/1000 mL * 1 moll/1 L * 1 mol/1000 mmol = 1*10^-4 mol NaOH 1 mL * 1 L/1000 mL * 1 mol/1 L * 1 mol/1000 mmol = 1*10^-3 mol NaOH Mol of NaOH - Mol of HAuCl4 = Mol of OH 1. 0 2. 2.5*10^-6 mol 3. 9.75*10^-5 mol 4. 9.975*10^-4 mol Concentration = Mol NaOH/ 10mL * 1000mL/ 1 L pH = -log(concentration) Ratio 1:1:0.5 --> pH 7 Ratio 1:1:1 --> pH 10.4 Ratio 1:1:0.1 stock --> pH 11.9 Ratio 1:1:1 stock --> pH 13 Titration Stock Solution Prep (0.0200 L AA)*(0.000250 mol/L AA)*(1 mol NaOH/mol AA)/(0.0200 L NaOH) = 0.000250 M NaOH M1V1 = M2V2 500mL * 2.5*10^-4 M NaOH = x * 1M NaOH ------> x = 0.125mL Data Amino Acid Solutions prepared last week did not work at any concentration. Gold Nanoparticle and Nano fiber solutions prepared with BSA From left to right Au:BSA ratio: 60, 90, 120, 150 Nano particles made in 60, 90, and 120. Nano fibers made in 120 and 150. 90 darker than 60. Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.