User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/03

Applied Soil Microbial Ecology

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Remains of the Day Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 Forward primer - sent as 1 - 32 in the order they were run on the below gel (remember it was multichannel so 1-8 from the first strip and staggered with 9-16 and etc). Cut potatoes for innoculation with the rest of the endophytes tommorow. Found good article about induction of SAR with innoculation with Methylobacteria (http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046802#pone.0046802-Rosenblueth1)