Found reference that gave me the NifH TRFLP primers I have: "Effect of N-fertilization, plant genotype and environmental conditions on nifH gene pools in roots of rice"
Did 96 well plate read of potato endophytes growing on nitrogen free LGI
George suggests I go ahead and sequence all the endophytes that I isolated from potatoes
Isolated DNA from 26 more frozen growth room tomatoe samples - 1 more day to go, then I start on the rhizosphere