User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/05/08

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Remains of the Day

  • Photograph and harvest the rest of the mature tomato plants
  • Submit project sheets for the TRFLP of the strawberry samples
  • Order more Zymo PCR cleanup kits
  • Order more 96 well plates and aluminium foil sealer
  • Run gel of PCR from last night, column purify today's and yesterday's PCR, then restrict with BstNI - as seen below, some reactions need to be redone this aft
  • Extracted tomato root and rhizosphere DNA was later adjusted to 30 ng/ul and put into a 96 well plate for freezing/storage


Soil TreatmentGenotype Shoot Weight Root Weight
Autoclaved sand Heinz 2401 2.25 3.67
Autoclaved sand Heinz 2401 5.56 6.17
Autoclaved sand Heinz 2401 3.36 4.49
Autoclaved sand Heinz 2401 3.88 5.49
Autoclaved sand Heinz 2401 4.53 4.96
1% Molasses TVD Soil Heinz 2401 3.81 1.78
1% Molasses TVD Soil Heinz 2401 4.71 0.95
1% Molasses TVD Soil Heinz 2401 4.82 1.42
1% Molasses TVD Soil Heinz 2401 3.19 1.06
1% Molasses TVD Soil Heinz 2401 2.62 1.30
Air Dried TVD Soil RST 3.46 1.78
Air Dried TVD Soil RST 3.01 2.09
Air Dried TVD Soil RST 2.38 2.08
Air Dried TVD Soil RST 3.00 2.41
Air Dried TVD Soil RST 1.82 1.20
Air Dried TVD Soil Beaufort 4.92 2.70
Air Dried TVD Soil Beaufort 4.39 2.31
Air Dried TVD Soil Beaufort 3.67 2.00
Air Dried TVD Soil LA1777 6.25 2.17
Air Dried TVD Soil LA1777 3.79 1.08
Air Dried TVD Soil LA1777 5.32 2.46
Air Dried TVD Soil LA1777 3.38 0.62


Image:May8,2012GEL.tif



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