User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/01/18

Applied Soil Microbial Ecology

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Remains of the Day Run gel of gradient PCRs Redo gradient PCR of healthy/unhealthy soil using half the primer (did at 50, 55, 60 degrees with either 10 or 20 pmol each of primer - also did some 10 at 52 degrees for TRFLP comparison to 799F/1492R) Rafiq talk at 10 Do ethanol precip of PCRs (including my PCRs with my primers) and start TRFLP digestion trial Harvest potato leaf tissue in liquid nitrogen Great article about PCR bias: http://www.nature.com/ismej/journal/v3/n12/full/ismej200989a.html#fig3 And another one critisizing 63F - http://onlinelibrary.wiley.com/doi/10.1016/S0378-1097%2803%2900218-0/pdf