User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/09/01

Maize Endophyte Biofertilizers

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The Daily Grind There's a problem with acetoin production in E. coli at the levels the PSBA promoter at high copy number induces: no cells survive. Therefore I better try this using an inducible promoter like the arabinose one. Screening alsD in pSB1A2 colonies for positives using PCR Sending some samples for sequencing including Norma's BRP Consolidating all the clean colonies for plate 3 glycerol stock and for slow growing plate 4 Making plates for biochemical screen and stamping them at the end of the day before freezing the glycerols