χρόνος πέρασμα May 25th 2011
- Today I checked the petri dishes that were left incubating. They all were contaminated. Miguel told us what problems could have been the culprits. Anyway, Fabricio López and I will start it over again.
- I let incubating at 37° the bacteria DH5α carrying the plasmid pBBRMCS5 in two test tubes with 5ml of liquid LB medium. 1 test tube contains only gentamicin, without bacteria, as a negative control. All test tubes were added with 5µl of gentamicin.
- I used the thermocycler to amplify the amplification product Pablo García did (tube #2).
Tube A
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Tube B
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Tube C
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27.5 µl
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27.5 µl
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27.5 µl
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H20
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10 µl
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10 µl
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10 µl
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DNA Polymerase Phusion Buffer
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1 µl
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1 µl
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1 µl
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dNTPs
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5 µl
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5 µl
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5 µl
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Oligo Forward
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5 µl
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5 µl
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5 µl
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Oligo Reverse
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1 µl
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1 µl
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0 µl
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DNA template (Pablo's PCR tube #2)
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0.5µl
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0.5µl
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0.5µl
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DNA Polymerase Phusion
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50 µl
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50 µl
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49 µl
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Total
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- Tube C is a negative control.
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