User:Daniel B Rosen/Notebook/Methylation Sensor/2011/10/12
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Daniel B Rosen 20:48, 12 October 2011 (EDT)Finish Maxi Prep ppGFP plasmids (2,4,6,8)Blank TE Sample 2, reran
In Silico Build of mCherry plasmids (9,10,11,12)Digest Confirmation of mCherry PlasmidsPassage 293T cells (1x15 cm --> 2x; 1x 15 cm --> 2x6well)note: 2 6 wells prepared with collagenase yesterday 6.94E6 cells/mL seeded at 2mL/well in 6 well plates (2x) 5.85E6 cells/mL seeded at 2.825E6 cells/mL at 25mL/plate in 15 cm dish (2x) |