User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/29

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PCR amplification of chromatin remodeling Reagent Volume Expected: 1. ANM5 = 1911, 2. MYB = 159,1764 3. Carm1 = 1827 4. SETD7 = 459,642 5. KMT2E = 1176,4401 6. SETD1A = 420,4704 Today's gel 15 μL/lane; 1% agarose; Ladder DNA(plasmid) 1.0 μL 1X GoTaq 12.5 F primer 1.0 R Primer 1.0 dH2O 9.5   25 μL Transforming P300 plasmids Transformation protocol Sample ID Expected Observed colonies PBS P300 Growth None 0.00 NHA P300 Growth None 0.00 CHA P300 Growth None 0.00 Plasmids were sent on filter paper and is possible did not remain intact during transit.