Confirm The BSMB1 Removal
Plasmid Type |
SD-BD002 1 |
SD-BD002 |
SD-BD002 |
BD002
|
Plasmid DNA |
3.0 μl |
3.0 μl |
3.0 μl |
3.0 μl
|
BSMB1 |
1.0 μl |
0.0 μL |
0.0 μL |
1.0 μL
|
Spe1 |
0.0 μl |
1.0 μL |
0.0 μL |
1.0μL
|
10x FastDigest buffer + green loading dye |
1.5 μl |
1.5 μl |
1.5 μl |
1.5 μl
|
dH2O |
9.5 μl |
9.5 μL |
10.5 μL |
9.5 μL
|
|
15.0 μl total |
15μL |
15μL |
15μL
|
Incubate at 37°C for 10 minutes.
|
1- SD-BD002 means BD002 plasmid after the Site-Directed Mutagenesis.
- Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.
|