18/12/2012

RFP Plate Reader Serial Dilution

1. Transfer 100 μl of RFP from stock to 100μl DI water. (50% solution)
2. Transfer 100 μl of 50% solution to 100 μl DI water. (25% solution)
3. Transfer 100 μl of 25% solution to 100 μl DI water. (12.5% solution)
4. Transfer 100 μl of 12.5% solution to 100 μl DI water. (6.25% solution)

Plate Reader

• Transfer 50 μl of each solution and a control to the Costar 96 clear bottom black side.
• Here is the software setting:
  • Fluorescence
  • Endpoint
  • Full Plate
  • Excitation: 540, Emission: 600
  • Optics: Top
  • Gain: AutoScale
  • Light Source: Xenon Flash, Lamp Energy: High
  • Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10
  • Read Height: 9.5 mm
• Click OK and then Run the experiment.
• Export the data to an excel file.
• The software will deduce the control data from the sample data

Result