User:Ashish Bhattarai/Notebook/EBC 571/2017/09/19

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Bradford Analysis:

Bradford assay is one of the ways to calculate the concentration of protein. One of the proteins that we have been using so far in this lab is BSA. A 50mL stock solution of BSA 5mg/5mLsolution is prepared. The actual solution concentration is calculated.

Actual solution concentration = 1014.8ug/ml

Next, 6 standard solutions between 1ug/ml and 20ug/ml of BSA is made.