I cleaned up the PCR reaction from Thursday and the nanodrop revealed:
- 227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA)
Since I got a new bottom oligo with 5' biotinylation I will have to anneal a new adapter duplex.
I only have SappBR so I will need to ligate the adapter to here first and then ligate this to the digested pALS.
- 89ng/ul --> ~31nM -- ~2.7ug
- 151.1ng/ul --> ~57nM -- ~4.5ng