User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/02

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Purpose and Design After finishing all of the required UV-Vis runs, the research group designed a series of control experiments. The same UV-Vis experiments were re-run without any BSA being added to the solution. The purpose of this is to evaluate whether nanoparticles will form solely in the presence of fructose and to determine the effect of fructose on formation at all pHs and all concentrations of fructose (0.0625, 0.125, 0.25, 0.75, and 1.25 mM Fructose). Sample Preparation Samples were prepared as follows. Note that there was no BSA added to any of the samples. It should be noted that nanoparticles that did form at pH 11 were in a very pink solution. Previous solutions were more purple. This image shows the formation of nanoparticles for 0.0625, 0.125, 0.25, and 0.75 mM Fructose. At all concentrations, nanoparticles only formed at pH 11. This image shows the formation of nanoparticles for 1.25 mM Fructose. Nanoparticles only formed at pH 11. The above image illustrates the different pink vs. purple color of the nanoparticles produced with and without BSA. The vial on the left is 1.25 mM Fructose with 3.125 uM BSA (darker purple). The vial on the right contains 1.25 mM Fructose with no BSA (lighter pink). The difference in color suggests that BSA has an impact on the production of Au NPs at pH 11. Both are at pH 11 because nanoparticles were only formed by the BSA-less solutions at pH 11. Data Analysis