User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/10/05

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Sample Preparation

  • Samples were not run with the heater turned on on 161005. The samples were redone on 161006.

BSA Ocean Optics Final Sample Solution Concentrations:

  1. 3 mL total
  2. 0.25 mM Au
  3. 3.125 μM BSA
  4. HCl or NaOH appropriate for your pH (7 today)

Stock solution Concentrations:

  1. 3.73 mM AuCl3
  2. 46.5 mM BSA
  3. 1 mM HCl
  4. DI HOH

Solution Transfer Volumes:

  1. 2597 uL HOH
  2. 201.6 uL AuCl3
  3. 201.0 uL BSA
  4. 0.3 uL HCl

Results

Image:Oceanoptics161006pH7BSA.PNG

The above chart shows the UV-Vis maximum absorbance over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past.

Image:Oceanoptics161006pH7BSAA530.PNG

The above chart shows the UV-Vis A(535.15) over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past, but there is still a trend showing an increase over time.

Image:Oceanoptics161006pH7BSAWAVELENGTH.PNG

The chart above shows an increase in the maximum wavelength around 7000 sec. The readings making up this chart are much "shakier" than previous runs.

  • We may need to redo this run.


Personal tools