User:Anh Do/Notebook/CHEM 481 Polymer/2017/03/03

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Task List Wet Sieving beads (cont.) Standardize dye solution Create buffer solution for beads Buffer solution for beads For acid exchange of beads, the beads took a long time to reach equilibrium when using pH=1 => Use higher pH = 3, pH = 5, pH = 7 Formula for creating citrate buffer was based on this link and this link Create 140 mL 0.1M citric acid solution Mass of citric acid (M.wt = 210.12 g/mol) = (210.12 g/mol)*(0.1mol/L)*(140mL/1000mL/L) = 2.9414 g Create 85 mL 0.1M trisodium citrate Mass of trisodium citrate (M.wt = 294.14 g/mol) = (194.14 g/mol)*(0.1mol/L)*(85mL/1000mL/L) = 2.50019 g Create 90 mL 0.2M dibasic sodium phosphate Mass of dibasic sodium phosphate (M.wt = 141.96 g/mol) = (141.96 g/mol)*(0.2mol/L)*(90mL/1000mL/L) = 2.55528 g pH = 3 citrate buffer (100 mL): Volume of 0.1M citric acid: 82.0 mL Volume of 0.1M trisodium citrate: 18.0 mL pH = 5 citrate buffer (100 mL): Volume of 0.1M citric acid: 35.0 mL Volume of 0.1M trisodium citrate: 65.0 mL pH = 7 phosphate-citrate buffer (100 mL): Volume of 0.1M citric acid: 13 mL Volume of 0.2M dibasic sodium phosphate: 87mL